25-HVD3 25HVD3 Calcifediol Calcidiol H-VD3 25-Hydroxycholecalciferol 25-Hydroxy Vitamin D3 25(OH)DELK Biotechnology HVD3(25-Hydroxyvitamin D3) ELISA Kit

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Product Name ELK Biotechnology HVD3(25-Hydroxyvitamin D3) ELISA Kit
Catalog No. ELK7890
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of General 25-Hydroxyvitamin D3 concentrations in serum, plasma and other biological fluids
Features
Sensitivity: 0.91 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;

Alternate Names: 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.