InsP3 Inositol 1,4,5-Trisphosphate TriphosphoinositolELK Biotechnology IP3(Inositol Triphosphate) ELISA Kit

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Product Name ELK Biotechnology IP3(Inositol Triphosphate) ELISA Kit
Catalog No. ELK8148
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of General Inositol Triphosphate concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 49.3 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Signal transduction;Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Bone metabolism;

Alternate Names: InsP3; Inositol 1,4,5-Trisphosphate; Triphosphoinositol

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of IP3 in the samples is then determined by comparing the OD of the samples to the standard curve.