BuChE CHE1 E1 Pseudocholinesterase Cholinesterase Acylcholine acylhydrolase Butyrylcholine esterase Choline esterase IIELK Biotechnology Mouse BCHE(Butyrylcholinesterase) ELISA Kit

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Product Name ELK Biotechnology Mouse BCHE(Butyrylcholinesterase) ELISA Kit
Catalog No. ELK7806
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Mouse Butyrylcholinesterase concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 0.87 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Enzyme & Kinase;Metabolic pathway;Neuro science;

Alternate Names: BuChE; CHE1; E1; Pseudocholinesterase; Cholinesterase; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse BCHE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BCHE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse BCHE in the samples is then determined by comparing the OD of the samples to the standard curve.