Acidic mammalian chitinase [Precursor] Acidic mammalian chitinase AMCase Chia CHIA_HUMAN CHIT2 chitinase, acidic DKFZp313J1722 ECF L Lung specific protein TSA1902 Lung-specific protein TSA1902 RP5 1125M8.1 TSA1902ELK Biotechnology Mouse Chia(Acidic mammalian chitinase) ELISA Kit

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Product Name ELK Biotechnology Mouse Chia(Acidic mammalian chitinase) ELISA Kit
Catalog No. ELK9754
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Mouse Acidic mammalian chitinase concentrations in serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Immunology; Microbiology; Signal Transduction

Alternate Names: Acidic mammalian chitinase [Precursor]; Acidic mammalian chitinase; AMCase; Chia; CHIA_HUMAN; CHIT2; chitinase, acidic; DKFZp313J1722; ECF L; Lung specific protein TSA1902; Lung-specific protein TSA1902; RP5 1125M8.1; TSA1902

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Chia. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Chia. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Chia, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse Chia in the samples is then determined by comparing the OD of the samples to the standard curve.