COL7-A1 COL7A1 EBD1 EBDCT EBR1 Long-chain collagen Epidermolysis Bullosa,Dystrophic,Dominant And Recessive Collagen Alpha-1(VII)chainELK Biotechnology Mouse COL7(Collagen Type VII) ELISA Kit

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Product Name ELK Biotechnology Mouse COL7(Collagen Type VII) ELISA Kit
Catalog No. ELK6603
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Mouse Collagen Type VII concentrations in Serum, plasma and other biological fluids.
Features
Sensitivity: 0.106 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism;

Alternate Names: COL7-A1; COL7A1; EBD1; EBDCT; EBR1; Long-chain collagen; Epidermolysis Bullosa,Dystrophic,Dominant And Recessive; Collagen Alpha-1(VII)chain

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse COL7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse COL7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse COL7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse COL7 in the samples is then determined by comparing the OD of the samples to the standard curve.