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Product Information

  • Product Name ELK Biotechnology Mouse IAPP(Islet Amyloid Polypeptide) ELISA Kit
  • Catalog No. ELK4946
  • Pack Size 96T/48T
  • Brand ELK Biotechnology Co. Ltd.
  • Product Detail This ELISA kit applies to the in vitro quantitative determination of Mouse Islet Amyloid Polypeptide concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Features

    Sensitivity: 2.74 pg/mL

    Standard: 500 pg/mL

    Detection Range: 7.82-500 pg/mL

    Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Metabolic pathway;Endocrinology;Hepatology;

Alternate Names: IAP; DAP; Amylin; Insulinoma Amyloid Polypeptide; Diabetes-associated peptide

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse IAPP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IAPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse IAPP in the samples is then determined by comparing the OD of the samples to the standard curve.

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