LAL CESD Lipase A,Lysosomal Acid,Cholesterol Esterase(Wolman Disease) Acid Cholesteryl Ester Hydrolase Lysosomal acid lipase/cholesteryl ester hydrolaseELK Biotechnology Mouse LIPA(Lipase A, Lysosomal Acid) ELISA Kit

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Product Name ELK Biotechnology Mouse LIPA(Lipase A, Lysosomal Acid) ELISA Kit
Catalog No. ELK7101
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Mouse Lipase A, Lysosomal Acid concentrations in serum, plasma, tissue homogenates and other biological fluids
Features
Sensitivity: 0.143 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Enzyme & Kinase;

Alternate Names: LAL; CESD; Lipase A,Lysosomal Acid,Cholesterol Esterase(Wolman Disease); Acid Cholesteryl Ester Hydrolase; Lysosomal acid lipase/cholesteryl ester hydrolase

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LIPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LIPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LIPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse LIPA in the samples is then determined by comparing the OD of the samples to the standard curve.