NR1-H3 LXR-A LXRA RLD-1 Nuclear Receptor Subfamily 1,Group H,Member 3 Oxysterols receptor LXR-alphaELK Biotechnology Mouse LXRa(Liver X Receptor Alpha) ELISA Kit

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Product Name ELK Biotechnology Mouse LXRa(Liver X Receptor Alpha) ELISA Kit
Catalog No. ELK6666
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Mouse Liver X Receptor Alpha concentrations in Tissue homogenates and other biological fluids.
Features
Sensitivity: 0.103 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hepatology;

Alternate Names: NR1-H3; LXR-A; LXRA; RLD-1; Nuclear Receptor Subfamily 1,Group H,Member 3; Oxysterols receptor LXR-alpha

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LXRa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LXRa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LXRa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse LXRa in the samples is then determined by comparing the OD of the samples to the standard curve.