GS1 MYH12 MYO5 MYOXIN MYR12 myosin V myosin Va Myosin,Heavy Polypeptide Kinase Myosin Heavy Chain 12 Myoxin Myosin V Dilute myosin heavy chain, non-muscleELK Biotechnology Mouse MYO5A(Myosin VA) ELISA Kit

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Product Name ELK Biotechnology Mouse MYO5A(Myosin VA) ELISA Kit
Catalog No. ELK6050
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Mouse Myosin VA concentrations in Tissue homogenates and other biological fluids.
Features
Sensitivity: 0.045 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Enzyme & Kinase;

Alternate Names: GS1; MYH12; MYO5; MYOXIN; MYR12; myosin V; myosin Va; Myosin,Heavy Polypeptide Kinase; Myosin Heavy Chain 12; Myoxin; Myosin V; Dilute myosin heavy chain, non-muscle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MYO5A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MYO5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MYO5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse MYO5A in the samples is then determined by comparing the OD of the samples to the standard curve.