P4HB ERBA2L PDIA1 PO4DB PROHB, DSI, GIT, PDI, PO4HB, P4Hbeta Prolyl 4-Hydroxylase Subunit Beta Procollagen-Proline 2-Oxoglutarate 4-Dioxygenase Beta PolypeptideELK Biotechnology Mouse PDI(Protein Disulfide Isomerase) ELISA Kit

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Product Name ELK Biotechnology Mouse PDI(Protein Disulfide Isomerase) ELISA Kit
Catalog No. ELK8856
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Mouse Protein Disulfide Isomerase concentrations in serum, plasma, tissue homogenates and other biological fluids
Features
Sensitivity: 0.105 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Enzyme & Kinase;

Alternate Names: P4HB; ERBA2L; PDIA1; PO4DB; PROHB, DSI, GIT, PDI, PO4HB, P4Hbeta; Prolyl 4-Hydroxylase Subunit Beta; Procollagen-Proline 2-Oxoglutarate 4-Dioxygenase Beta Polypeptide

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PDI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PDI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PDI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse PDI in the samples is then determined by comparing the OD of the samples to the standard curve.