FAAR NR1C2 NUC1 NUCI PPARB PPAR?/B Nuclear Receptor Subfamily 1,Group C,Member 2 Nuclear hormone receptor 1 Peroxisome proliferator-activated receptor betaELK Biotechnology Mouse PPARd(Peroxisome Proliferator Activated Receptor Delta) ELISA Kit

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Product Name ELK Biotechnology Mouse PPARd(Peroxisome Proliferator Activated Receptor Delta) ELISA Kit
Catalog No. ELK6907
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Mouse Peroxisome Proliferator Activated Receptor Delta concentrations in tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;Metabolic pathway;Tumor immunity;

Alternate Names: FAAR; NR1C2; NUC1; NUCI; PPARB; PPAR?/B; Nuclear Receptor Subfamily 1,Group C,Member 2; Nuclear hormone receptor 1; Peroxisome proliferator-activated receptor beta

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PPARd. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PPARd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PPARd, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse PPARd in the samples is then determined by comparing the OD of the samples to the standard curve.