CLN2 GIG1 LPIC Tripeptidyl aminopeptidase Lysosomal pepstatin-insensitive protease Ceroid-Lipofuscinosis,Neuronal 2,Late Infantile Cell growth-inhibiting gene 1 proteinELK Biotechnology Mouse TPP1(Tripeptidyl Peptidase I) ELISA Kit

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Product Name ELK Biotechnology Mouse TPP1(Tripeptidyl Peptidase I) ELISA Kit
Catalog No. ELK6821
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Mouse Tripeptidyl Peptidase I concentrations in Tissue homogenates and other biological fluids.
Features
Sensitivity: 0.055 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Enzyme & Kinase;

Alternate Names: CLN2; GIG1; LPIC; Tripeptidyl aminopeptidase; Lysosomal pepstatin-insensitive protease; Ceroid-Lipofuscinosis,Neuronal 2,Late Infantile; Cell growth-inhibiting gene 1 protein

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TPP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TPP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TPP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Mouse TPP1 in the samples is then determined by comparing the OD of the samples to the standard curve.