Assay Type: Sandwich
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Developmental science;Bone metabolism;
Alternate Names: PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3; PPARg; Peroxisome Proliferator Activated Receptor Gamma
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit PPAR-?. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit PPAR-?. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit PPAR-?, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rabbit PPAR-? in the samples is then determined by comparing the OD of the samples to the standard curve.
