CD156b TACE CSVP Tumor Necrosis Factor,Alpha,Converting Enzyme Disintegrin and metalloproteinase domain-containing protein 17 Snake venom-like proteaseELK Biotechnology Rat ADAM17(A Disintegrin And Metalloprotease 17) ELISA Kit

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Product Name ELK Biotechnology Rat ADAM17(A Disintegrin And Metalloprotease 17) ELISA Kit
Catalog No. ELK6759
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat A Disintegrin And Metalloprotease 17 concentrations in Tissue homogenates, cell lysates and other biological fluids
Features
Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Enzyme & Kinase;Tumor immunity;

Alternate Names: CD156b; TACE; CSVP; Tumor Necrosis Factor,Alpha,Converting Enzyme; Disintegrin and metalloproteinase domain-containing protein 17; Snake venom-like protease

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADAM17. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ADAM17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ADAM17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat ADAM17 in the samples is then determined by comparing the OD of the samples to the standard curve.