Autophagy-related protein 3, Preconditioning-inducible gene 1 protein, APG3-like, Apg3lELK Biotechnology Rat ATG3(Autophagy Related Protein 3) ELISA Kit

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Product Name ELK Biotechnology Rat ATG3(Autophagy Related Protein 3) ELISA Kit
Catalog No. ELK0556
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Autophagy Related Protein 3 concentrations in tissue homogenates, cell lysates and other biological fluids
Features
Sensitivity: 0.14 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Metabolic pathway;

Alternate Names: Autophagy-related protein 3, Preconditioning-inducible gene 1 protein, APG3-like, Apg3l

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ATG3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ATG3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ATG3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat ATG3 in the samples is then determined by comparing the OD of the samples to the standard curve.