NR1I3 NR1-I3 CAR1 MB67 Nuclear Receptor Subfamily 1,Group I,Member 3 Constitutive active response Constitutive activator of retinoid response Orphan nuclear receptor MB67ELK Biotechnology Rat CAR(Constitutive Androstane Receptor) ELISA Kit

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Product Name ELK Biotechnology Rat CAR(Constitutive Androstane Receptor) ELISA Kit
Catalog No. ELK7571
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Constitutive Androstane Receptor concentrations in Tissue homogenates, cell lysates and other biological fluids
Features
Sensitivity: 0.064 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;Metabolic pathway;Endocrinology;

Alternate Names: NR1I3; NR1-I3; CAR1; MB67; Nuclear Receptor Subfamily 1,Group I,Member 3; Constitutive active response; Constitutive activator of retinoid response; Orphan nuclear receptor MB67

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CAR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CAR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat CAR in the samples is then determined by comparing the OD of the samples to the standard curve.