CP1B GLC3A Cytochrome P450,Family 1,Subfamily B,Polypeptide 1 Glaucoma 3,Primary InfantileELK Biotechnology Rat CYP1B1(Cytochrome P450 1B1) ELISA Kit

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Product Name ELK Biotechnology Rat CYP1B1(Cytochrome P450 1B1) ELISA Kit
Catalog No. ELK7418
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Cytochrome P450 1B1 concentrations in Tissue homogenates, cell lysates and other biological fluids
Features
Sensitivity: 0.31 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Metabolic pathway;

Alternate Names: CP1B; GLC3A; Cytochrome P450,Family 1,Subfamily B,Polypeptide 1; Glaucoma 3,Primary Infantile

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CYP1B1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CYP1B1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CYP1B1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat CYP1B1 in the samples is then determined by comparing the OD of the samples to the standard curve.