SLC6A3 DAT1 Sodium-dependent dopamine transporter Dopamine Active Transporter SLC6-A3 Solute Carrier Family 6(neurotransmitter Transporter,Dopamine),Member 3ELK Biotechnology Rat DAT(Dopamine Transporter) ELISA Kit

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Product Name ELK Biotechnology Rat DAT(Dopamine Transporter) ELISA Kit
Catalog No. ELK5654
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Dopamine Transporter concentrations in Tissue homogenates, cell lysates and other biological fluids
Features
Sensitivity: 0.117 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;Neuro science;

Alternate Names: SLC6A3; DAT1; Sodium-dependent dopamine transporter; Dopamine Active Transporter; SLC6-A3; Solute Carrier Family 6(neurotransmitter Transporter,Dopamine),Member 3

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DAT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat DAT in the samples is then determined by comparing the OD of the samples to the standard curve.