Assay Type: Sandwich
Research Area: Enzyme & Kinase;
Alternate Names: DLD; Diaphorase; DLD; DLDD; DLDH; GCSL; LAD; Lipoamide Dehydrogenase; PHE3; 2-oxo-glutaRate complex; branched chain keto acid dehydrogenase complex; branched chain keto acid dehydrogenase complex; dihydrolipoamide dehydrogenase; E3 component of pyruvate dehydrogenase complex; dihydrolipoamide dehydrogenasemitochondrial; DLDH; E3; E3 component of pyruvate dehydrogenase complex; 2-oxo-glutaRate complex; EC 1.8.1; EC 1.8.1.4; GCSL; Glycine cleavage system L protein; glycine cleavage system protein L; lipoamide dehydrogenase; lipoamide reductase; lipoyl dehydrogenase
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DLD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DLD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DLD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat DLD in the samples is then determined by comparing the OD of the samples to the standard curve.
