KGF HBGF7 Keratinocyte Growth Factor Heparin-binding growth factor 7ELK Biotechnology Rat FGF7(Fibroblast Growth Factor 7) ELISA Kit

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Product Name ELK Biotechnology Rat FGF7(Fibroblast Growth Factor 7) ELISA Kit
Catalog No. ELK5234
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Fibroblast Growth Factor 7 concentrations in Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 5.37 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Cytokine;Tumor immunity;Infection immunity;Developmental science;Pulmonology;Dermatology;

Alternate Names: KGF; HBGF7; Keratinocyte Growth Factor; Heparin-binding growth factor 7

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat FGF7 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FGF7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat FGF7 in the samples is then determined by comparing the OD of the samples to the standard curve.