NR1H4 BAR FXR HRR1 RIP14 Nuclear Receptor Subfamily 1,Group H,Member 4 Bile acid receptor Farnesol receptor HRR-1 Retinoid X receptor-interacting protein 14ELK Biotechnology Rat FXR(Farnesoid X Receptor) ELISA Kit

Add to Enquire list

Product Name ELK Biotechnology Rat FXR(Farnesoid X Receptor) ELISA Kit
Catalog No. ELK6978
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Farnesoid X Receptor concentrations in Tissue homogenates, cell lysates and other biological fluids
Features
Sensitivity: 0.053 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;Metabolic pathway;Endocrinology;Hormone metabolism;

Alternate Names: NR1H4; BAR; FXR; HRR1; RIP14; Nuclear Receptor Subfamily 1,Group H,Member 4; Bile acid receptor; Farnesol receptor HRR-1; Retinoid X receptor-interacting protein 14

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FXR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FXR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FXR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat FXR in the samples is then determined by comparing the OD of the samples to the standard curve.