MTLRP Preproghrelin Growth Hormone Secretagogue Receptor Ligand Appetite-regulating hormone Growth hormone-releasing peptide Motilin-related peptideELK Biotechnology Rat GHRL(Ghrelin) ELISA Kit

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Product Name ELK Biotechnology Rat GHRL(Ghrelin) ELISA Kit
Catalog No. ELK6912
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Ghrelin concentrations in Serum, plasma and other biological fluids.
Features
Sensitivity: 10.18 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Endocrinology;

Alternate Names: MTLRP; Preproghrelin; Growth Hormone Secretagogue Receptor Ligand; Appetite-regulating hormone; Growth hormone-releasing peptide; Motilin-related peptide

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GHRL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.