SLC2A4 Solute carrier family 2, facilitated glucose transporter member 4 Glucose transporter type 4, insulin-responsiveELK Biotechnology Rat GLUT4(Glucose Transporter 4) ELISA Kit

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Product Name ELK Biotechnology Rat GLUT4(Glucose Transporter 4) ELISA Kit
Catalog No. ELK5796
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Glucose Transporter 4 concentrations in tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 0.063 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;Metabolic pathway;Endocrinology;Cardiovascular biology;

Alternate Names: SLC2A4; Solute carrier family 2, facilitated glucose transporter member 4; Glucose transporter type 4, insulin-responsive

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GLUT4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GLUT4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GLUT4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat GLUT4 in the samples is then determined by comparing the OD of the samples to the standard curve.