SLC15A1 HPECT1 PEPT1 Solute Carrier Family 15 Member 1,Oligopeptide Transporter Oligopeptide transporter, small intestine isoform Intestinal H(+)/peptide cotransporterELK Biotechnology Rat HPEPT1(Hydrogen Ion/Peptide Transporter 1) ELISA Kit

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Product Name ELK Biotechnology Rat HPEPT1(Hydrogen Ion/Peptide Transporter 1) ELISA Kit
Catalog No. ELK7825
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Hydrogen Ion/Peptide Transporter 1 concentrations in tissue homogenates, cell lysates and other biological fluids
Features
Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;

Alternate Names: SLC15A1; HPECT1; PEPT1; Solute Carrier Family 15 Member 1,Oligopeptide Transporter; Oligopeptide transporter, small intestine isoform; Intestinal H(+)/peptide cotransporter

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HPEPT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HPEPT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HPEPT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat HPEPT1 in the samples is then determined by comparing the OD of the samples to the standard curve.