11DH 11-beta-HSD1 HDL HSD11 HSD11B HSD11L SDR26C1 Corticosteroid 11-beta-dehydrogenase isozyme 1 Short Chain Dehydrogenase/Reductase Family 26C,Member 1ELK Biotechnology Rat HSD11b1(11-Beta-Hydroxysteroid Dehydrogenase Type 1) ELISA Kit

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Product Name ELK Biotechnology Rat HSD11b1(11-Beta-Hydroxysteroid Dehydrogenase Type 1) ELISA Kit
Catalog No. ELK6356
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat 11-Beta-Hydroxysteroid Dehydrogenase Type 1 concentrations in Tissue homogenates, cell lysates and other biological fluids.
Features
Sensitivity: 0.124 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hepatology;Hormone metabolism;

Alternate Names: 11DH; 11-beta-HSD1; HDL; HSD11; HSD11B; HSD11L; SDR26C1; Corticosteroid 11-beta-dehydrogenase isozyme 1; Short Chain Dehydrogenase/Reductase Family 26C,Member 1

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HSD11b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HSD11b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HSD11b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat HSD11b1 in the samples is then determined by comparing the OD of the samples to the standard curve.