RLNL RLF Relaxin-Like Factor,Leydig Cell Leydig insulin-like peptideELK Biotechnology Rat INSL3(Insulin Like Protein 3) ELISA Kit

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Product Name ELK Biotechnology Rat INSL3(Insulin Like Protein 3) ELISA Kit
Catalog No. ELK6400
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Insulin Like Protein 3 concentrations in Serum, plasma and other biological fluids.
Features
Sensitivity: 62.8 pg/mL

Standard: 15000 pg/mL

Detection Range: 234.38-15000 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Endocrinology;

Alternate Names: RLNL; RLF; Relaxin-Like Factor,Leydig Cell; Leydig insulin-like peptide

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat INSL3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat INSL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat INSL3 in the samples is then determined by comparing the OD of the samples to the standard curve.