PLA2G7 PAF-AH PAFAH Lp-PLA2 LDL-PLA2 Platelet Activating Factor Acetylhydrolase,Plasma Phospholipase A2,Group VII LDL-associated phospholipase A2ELK Biotechnology Rat LpPLA2(Phospholipase A2, Lipoprotein Associated) ELISA Kit

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Product Name ELK Biotechnology Rat LpPLA2(Phospholipase A2, Lipoprotein Associated) ELISA Kit
Catalog No. ELK5217
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Phospholipase A2, Lipoprotein Associated concentrations in Serum, plasma, tissue homogenates and other biological fluids.
Features
Sensitivity: 5.1 pg/mL

Standard: 800 pg/mL

Detection Range: 12.5-800 pg/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Enzyme & Kinase;Metabolic pathway;Infection immunity;

Alternate Names: PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LpPLA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LpPLA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LpPLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat LpPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve.