PPHA PABA Peptide Hydrolase Endopeptidase-2 N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha PABA Peptide HydrolaseELK Biotechnology Rat MEP1a(Meprin A Alpha) ELISA Kit

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Product Name ELK Biotechnology Rat MEP1a(Meprin A Alpha) ELISA Kit
Catalog No. ELK5520
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Meprin A Alpha concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 15.2 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Metabolic pathway;

Alternate Names: PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MEP1a protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MEP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat MEP1a in the samples is then determined by comparing the OD of the samples to the standard curve.