MUC5-B MUC9 MG1 Oligomeric Mucus/Gel-Forming Mucin 5,Subtype B,Tracheobronchial Cervical mucin High molecular weight salivary mucin MG1 Sublingual gland mucinELK Biotechnology Rat MUC5B(Mucin 5 Subtype B) ELISA Kit

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Product Name ELK Biotechnology Rat MUC5B(Mucin 5 Subtype B) ELISA Kit
Catalog No. ELK5268
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Mucin 5 Subtype B concentrations in tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 0.119 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Tumor immunity;Infection immunity;Pulmonology;Dermatology;

Alternate Names: MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtype B,Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MUC5B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MUC5B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MUC5B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat MUC5B in the samples is then determined by comparing the OD of the samples to the standard curve.