Assay Type: Sandwich
Research Area: Cell Biology
Alternate Names: MuRF1 ; IRF ; RNF28 ; SMRZ ; TRIM63 ; IRFMURF2 ; Iris RING finger protein ; MuRF1 ; MURF1 ; MuRF-1 ; muscle specific ring finger protein 2 ; Muscle-specific RING finger protein 1 ; RING finger protein 28FLJ32380 ; RNF28E3 ubiquitin-protein ligase TRIM63 ; SMRZMURF-1 ; Striated muscle RING zinc finger protein ; tripartite motif containing 63 ; tripartite motif-containing 63 ; Tripartite motif-containing protein 63
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MuRF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MuRF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MuRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat MuRF1 in the samples is then determined by comparing the OD of the samples to the standard curve.
