SLC9A1 APNH Solute Carrier Family 9 Member 1,Sodium/Hydrogen Exchanger Antiporter,Na+/H+,Amiloride Sensitive Na(+)/H(+) antiporter, amiloride-sensitiveELK Biotechnology Rat NHE1(Sodium/Hydrogen Exchanger 1) ELISA Kit

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Product Name ELK Biotechnology Rat NHE1(Sodium/Hydrogen Exchanger 1) ELISA Kit
Catalog No. ELK0638
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Sodium/Hydrogen Exchanger 1 concentrations in serum, plasma, tissue homogenates and other biological fluids
Features
Sensitivity: 0.049 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;

Alternate Names: SLC9A1; APNH; Solute Carrier Family 9 Member 1,Sodium/Hydrogen Exchanger; Antiporter,Na+/H+,Amiloride Sensitive; Na(+)/H(+) antiporter, amiloride-sensitive

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NHE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NHE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NHE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat NHE1 in the samples is then determined by comparing the OD of the samples to the standard curve.