SPARC BM-40 Secreted Protein,Acidic,Cysteine-Rich Basement-membrane protein 40 Secreted protein acidic and rich in cysteineELK Biotechnology Rat ON(Osteonectin) ELISA Kit

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Product Name ELK Biotechnology Rat ON(Osteonectin) ELISA Kit
Catalog No. ELK1683
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Osteonectin concentrations in Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Features
Sensitivity: 0.249 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Metabolic pathway;Tumor immunity;Infection immunity;Hepatology;Bone metabolism;

Alternate Names: SPARC; BM-40; Secreted Protein,Acidic,Cysteine-Rich; Basement-membrane protein 40; Secreted protein acidic and rich in cysteine

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ON. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ON. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ON, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat ON in the samples is then determined by comparing the OD of the samples to the standard curve.