PDHCE1A PHE1A PHE1-A PDHA1 PDH-A Pyruvate Dehydrogenase(lipoamide)Alpha 1 Pyruvate Dehydrogenase E1 Component Subunit Alpha,Aomatic Form,MitochondrialELK Biotechnology Rat PDHa(Pyruvate Dehydrogenase Alpha) ELISA Kit

Add to Enquire list

Product Name ELK Biotechnology Rat PDHa(Pyruvate Dehydrogenase Alpha) ELISA Kit
Catalog No. ELK2703
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Pyruvate Dehydrogenase Alpha concentrations in tissue homogenates, cell lysates and other biological fluids
Features
Sensitivity: 0.29 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Enzyme & Kinase;Metabolic pathway;

Alternate Names: PDHCE1A; PHE1A; PHE1-A; PDHA1; PDH-A; Pyruvate Dehydrogenase(lipoamide)Alpha 1; Pyruvate Dehydrogenase E1 Component Subunit Alpha,Aomatic Form,Mitochondrial

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PDHa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PDHa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PDHa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat PDHa in the samples is then determined by comparing the OD of the samples to the standard curve.