CD243 ABCB1 ABC20 CLCS GP170 MDR1 PGY1 P-Glycoprotein Multidrug resistance protein 1 ATP-Binding Cassette,Sub-Family B(MDR/TAP),Member 1 Colchicin SensitivityELK Biotechnology Rat Pgp(Permeability Glycoprotein) ELISA Kit

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Product Name ELK Biotechnology Rat Pgp(Permeability Glycoprotein) ELISA Kit
Catalog No. ELK7569
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Permeability Glycoprotein concentrations in Tissue homogenates, cell lysates and other biological fluids
Features
Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;

Alternate Names: CD243; ABCB1; ABC20; CLCS; GP170; MDR1; PGY1; P-Glycoprotein; Multidrug resistance protein 1; ATP-Binding Cassette,Sub-Family B(MDR/TAP),Member 1; Colchicin Sensitivity

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Pgp. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Pgp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Pgp, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat Pgp in the samples is then determined by comparing the OD of the samples to the standard curve.