P3CP C-Propeptide Of Type III Procollagen Procollagen III Carboxy Terminal PropeptideELK Biotechnology Rat PIIICP(Procollagen III C-Terminal Propeptide) ELISA Kit

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Product Name ELK Biotechnology Rat PIIICP(Procollagen III C-Terminal Propeptide) ELISA Kit
Catalog No. ELK7684
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Procollagen III C-Terminal Propeptide concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Features
Sensitivity: 0.86 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Metabolic pathway;Bone metabolism;

Alternate Names: P3CP; C-Propeptide Of Type III Procollagen; Procollagen III Carboxy Terminal Propeptide

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PIIICP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PIIICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat PIIICP in the samples is then determined by comparing the OD of the samples to the standard curve.