PP2A PR53 PTPA Phosphotyrosyl Phosphatase Activator PP2A Phosphatase Activator PP2A, subunit B', PR53 isoform Serine/threonine-protein phosphatase 2A activatorELK Biotechnology Rat PPP2R4(Protein Phosphatase 2A Activator, Regulatory Subunit 4) ELISA Kit

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Product Name ELK Biotechnology Rat PPP2R4(Protein Phosphatase 2A Activator, Regulatory Subunit 4) ELISA Kit
Catalog No. ELK7162
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Protein Phosphatase 2A Activator, Regulatory Subunit 4 concentrations in Tissue homogenates and other biological fluids.
Features
Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Metabolic pathway;

Alternate Names: PP2A; PR53; PTPA; Phosphotyrosyl Phosphatase Activator; PP2A Phosphatase Activator; PP2A, subunit B', PR53 isoform; Serine/threonine-protein phosphatase 2A activator

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PPP2R4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PPP2R4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PPP2R4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat PPP2R4 in the samples is then determined by comparing the OD of the samples to the standard curve.