MBN PRTN3 ACPA AGP7 C-ANCA MBT P29 PRK Myeloblastin Serine Proteinase,Neutrophil,Wegener Granulomatosis Autoantigen Protease K Proteinase K Endopeptidase KELK Biotechnology Rat PR3(Proteinase 3) ELISA Kit

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Product Name ELK Biotechnology Rat PR3(Proteinase 3) ELISA Kit
Catalog No. ELK5641
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Proteinase 3 concentrations in Serum, plasma and other biological fluids.
Features
Sensitivity: 1.36 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Enzyme & Kinase;Infection immunity;Autoimmunity;

Alternate Names: MBN; PRTN3; ACPA; AGP7; C-ANCA; MBT; P29; PRK; Myeloblastin; Serine Proteinase,Neutrophil,Wegener Granulomatosis Autoantigen; Protease K; Proteinase K; Endopeptidase K

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PR3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat PR3 in the samples is then determined by comparing the OD of the samples to the standard curve.