NR1I2 SAR PAR1 PAR2 PRR BXR ONR1 PAR PARq SXR Nuclear Receptor Subfamily 1,Group I,Member 2 Steroid and xenobiotic receptor Pregnane X receptorELK Biotechnology Rat PXR(Pregnane X Receptor) ELISA Kit

Add to Enquire list

Product Name ELK Biotechnology Rat PXR(Pregnane X Receptor) ELISA Kit
Catalog No. ELK6436
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Pregnane X Receptor concentrations in Tissue homogenates, cell lysates and other biological fluids
Features
Sensitivity: 0.113 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;

Alternate Names: NR1I2; SAR; PAR1; PAR2; PRR; BXR; ONR1; PAR; PARq; SXR; Nuclear Receptor Subfamily 1,Group I,Member 2; Steroid and xenobiotic receptor; Pregnane X receptor

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PXR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PXR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PXR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat PXR in the samples is then determined by comparing the OD of the samples to the standard curve.