CD265 TNFRSF11A TNFRSF11-A ODFR OFE TRANCER Osteoclast differentiation factor receptor Tumor Necrosis Factor Receptor Superfamily,Member 11a,NFKB ActivatorELK Biotechnology Rat RANk(Receptor Activator Of Nuclear Factor Kappa B) ELISA Kit

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Product Name ELK Biotechnology Rat RANk(Receptor Activator Of Nuclear Factor Kappa B) ELISA Kit
Catalog No. ELK5786
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Receptor Activator Of Nuclear Factor Kappa B concentrations in Tissue homogenates, cell lysates and other biological fluids.
Features
Sensitivity: 0.06 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;Metabolic pathway;Bone metabolism;

Alternate Names: CD265; TNFRSF11A; TNFRSF11-A; ODFR; OFE; TRANCER; Osteoclast differentiation factor receptor; Tumor Necrosis Factor Receptor Superfamily,Member 11a,NFKB Activator

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat RANk. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat RANk. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat RANk, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat RANk in the samples is then determined by comparing the OD of the samples to the standard curve.