Rnase-A2 Rnase2 EDN Ribonuclease US Ribonuclease,RNase A Family,2 Liver,Eosinophil-Derived Neurotoxin MNon-secretory ribonucleaseELK Biotechnology Rat RNASE2(Ribonuclease A2) ELISA Kit

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Product Name ELK Biotechnology Rat RNASE2(Ribonuclease A2) ELISA Kit
Catalog No. ELK9258
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Ribonuclease A2 concentrations in Serum, plasma, tissue homogenates and other biological fluids
Features
Sensitivity: 0.128 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Enzyme & Kinase;

Alternate Names: Rnase-A2; Rnase2; EDN; Ribonuclease US; Ribonuclease,RNase A Family,2; Liver,Eosinophil-Derived Neurotoxin; MNon-secretory ribonuclease

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat RNASE2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat RNASE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat RNASE2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat RNASE2 in the samples is then determined by comparing the OD of the samples to the standard curve.