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Product Information

  • Product Name ELK Biotechnology Rat S100A10(S100 Calcium Binding Protein A10) ELISA Kit
  • Catalog No. ELK4262
  • Pack Size 96T/48T
  • Brand ELK Biotechnology Co. Ltd.
  • Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat S100 Calcium Binding Protein A10 concentrations in Tissue homogenates, cell lysates and other biological fluids.
  • Features

    Sensitivity: 0.115 ng/mL

    Standard: 20 ng/mL

    Detection Range: 0.32-20 ng/mL

    Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;Tumor immunity;Neuro science;

Alternate Names: ANX2L; ANX2LG; CAL1L; CLP11; GP11; P11; P10; Calpactin-1 light chain; Cellular ligand of annexin II; Annexin II Ligand,Calpactin I,Light Polypeptide

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat S100A10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat S100A10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat S100A10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat S100A10 in the samples is then determined by comparing the OD of the samples to the standard curve.

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