SECRET SEGN CALBL Calbindin-Like Secretagogin,EF-Hand Calcium Binding ProteinELK Biotechnology Rat SCGN(Secretagogin) ELISA Kit

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Product Name ELK Biotechnology Rat SCGN(Secretagogin) ELISA Kit
Catalog No. ELK6364
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Secretagogin concentrations in Serum, plasma and other biological fluids.
Features
Sensitivity: 24.7 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Metabolic pathway;

Alternate Names: SECRET; SEGN; CALBL; Calbindin-Like; Secretagogin,EF-Hand Calcium Binding Protein

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat SCGN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SCGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat SCGN in the samples is then determined by comparing the OD of the samples to the standard curve.