ENaCa ENaCalpha SCNEA SCNN1 Sodium Channel,Nonvoltage-Gated 1,Alpha Epithelial Na(+) channel subunit alpha Nonvoltage-gated sodium channel 1 subunit alphaELK Biotechnology Rat SCNN1a(Amiloride Sensitive Sodium Channel Subunit Alpha) ELISA Kit

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Product Name ELK Biotechnology Rat SCNN1a(Amiloride Sensitive Sodium Channel Subunit Alpha) ELISA Kit
Catalog No. ELK6807
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Amiloride Sensitive Sodium Channel Subunit Alpha concentrations in Serum, plasma, tissue homogenates and other biological fluids.
Features
Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;

Alternate Names: ENaCa; ENaCalpha; SCNEA; SCNN1; Sodium Channel,Nonvoltage-Gated 1,Alpha; Epithelial Na(+) channel subunit alpha; Nonvoltage-gated sodium channel 1 subunit alpha

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SCNN1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SCNN1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SCNN1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat SCNN1a in the samples is then determined by comparing the OD of the samples to the standard curve.