SEMAL Hsema-I Hsema-III SEMA1 SEMAD SEMAIII SemD Coll-1 Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3AELK Biotechnology Rat SEMA3A(Semaphorin 3A) ELISA Kit

Add to Enquire list

Product Name ELK Biotechnology Rat SEMA3A(Semaphorin 3A) ELISA Kit
Catalog No. ELK7966
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Semaphorin 3A concentrations in Serum, plasma, tissue homogenates and other biological fluids
Features
Sensitivity: 6.7 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: CD & Adhesion molecule;

Alternate Names: SEMAL; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SemD; Coll-1; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SEMA3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SEMA3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SEMA3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat SEMA3A in the samples is then determined by comparing the OD of the samples to the standard curve.