CD100 A8 BB18 GR3 M-Sema G M-Sema-G SEMAJ Coll-4 Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4DELK Biotechnology Rat SEMA4D(Semaphorin 4D) ELISA Kit

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Product Name ELK Biotechnology Rat SEMA4D(Semaphorin 4D) ELISA Kit
Catalog No. ELK9701
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Semaphorin 4D concentrations in serum, plasma, tissue homogenates and other biological fluids
Features
Sensitivity: 0.3 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;

Alternate Names: CD100; A8; BB18; GR3; M-Sema G; M-Sema-G; SEMAJ; Coll-4; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SEMA4D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SEMA4D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SEMA4D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat SEMA4D in the samples is then determined by comparing the OD of the samples to the standard curve.