THR-A NR1A1 AR7 EAR7 ERB-T1 ERBA1 NR1-A1 THRA1 THRA2 c-ERBA1 Erythroblastic Leukemia Viral Oncogene Homolog Nuclear Receptor Subfamily 1,Group A,Member 1ELK Biotechnology Rat THRa(Thyroid Hormone Receptor Alpha) ELISA Kit

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Product Name ELK Biotechnology Rat THRa(Thyroid Hormone Receptor Alpha) ELISA Kit
Catalog No. ELK6646
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Thyroid Hormone Receptor Alpha concentrations in Tissue homogenates and other biological fluids.
Features
Sensitivity: 0.268 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;Metabolic pathway;Endocrinology;Hormone metabolism;

Alternate Names: THR-A; NR1A1; AR7; EAR7; ERB-T1; ERBA1; NR1-A1; THRA1; THRA2; c-ERBA1; Erythroblastic Leukemia Viral Oncogene Homolog; Nuclear Receptor Subfamily 1,Group A,Member 1

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat THRa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat THRa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat THRa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat THRa in the samples is then determined by comparing the OD of the samples to the standard curve.