ERBA-BETA ERBA2 GRTH NR1A2 THR1 THRB1 THRB2 Erythroblastic Leukemia Viral(v-erb-a)Oncogene Homolog 2,Avian Nuclear Receptor Subfamily 1,Group A,Member 2ELK Biotechnology Rat THRb(Thyroid Hormone Receptor Beta) ELISA Kit

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Product Name ELK Biotechnology Rat THRb(Thyroid Hormone Receptor Beta) ELISA Kit
Catalog No. ELK5243
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Thyroid Hormone Receptor Beta concentrations in Tissue homogenates, cell lysates and other biological fluids
Features
Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Assay Length: 3.5h

Assay Type: Sandwich

Research Area: Signal transduction;Endocrinology;Hormone metabolism;

Alternate Names: ERBA-BETA; ERBA2; GRTH; NR1A2; THR1; THRB1; THRB2; Erythroblastic Leukemia Viral(v-erb-a)Oncogene Homolog 2,Avian; Nuclear Receptor Subfamily 1,Group A,Member 2

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat THRb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat THRb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat THRb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat THRb in the samples is then determined by comparing the OD of the samples to the standard curve.