TRF Thyrotropin-Releasing Factor Thyroliberin Protirelin Pro-tirelin Prothyroliberin TSH-releasing factorELK Biotechnology Rat TRH(Thyrotropin Releasing Hormone) ELISA Kit

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Product Name ELK Biotechnology Rat TRH(Thyrotropin Releasing Hormone) ELISA Kit
Catalog No. ELK2607
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of Rat Thyrotropin Releasing Hormone concentrations in serum, plasma and other biological fluids
Features
Sensitivity: 2.43 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Endocrinology;Neuro science;Hepatology;Hormone metabolism;

Alternate Names: TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Rat TRH in the samples is then determined by comparing the OD of the samples to the standard curve.