RT3 r-T3 Reverse T3 (2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid 3,3',5'-Triiodo-L-thyronineELK Biotechnology rT3(Reverse Triiodothyronine) ELISA Kit

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Product Name ELK Biotechnology rT3(Reverse Triiodothyronine) ELISA Kit
Catalog No. ELK7898
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of General Reverse Triiodothyronine concentrations in serum, plasma and other biological fluids
Features
Sensitivity: 27.55 pg/mL

Standard: 6000 pg/mL

Detection Range: 93.75-6000 pg/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Infection immunity;Endocrinology;Hormone metabolism;Autoimmunity;

Alternate Names: RT3; r-T3; Reverse T3; (2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rT3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to rT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve.