Ergocalciferol Deltalin Drisdol CalcidolELK Biotechnology VD2(Vitamin D2) ELISA Kit

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Product Name ELK Biotechnology VD2(Vitamin D2) ELISA Kit
Catalog No. ELK7870
Pack Size 96T/48T
Brand ELK Biotechnology Co. Ltd.
Product Detail This ELISA kit applies to the in vitro quantitative determination of General Vitamin D2 concentrations in Serum, plasma and other biological fluids.
Features
Sensitivity: 0.53 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Metabolic pathway;Neuro science;Nutrition metabolism;Bone metabolism;

Alternate Names: Ergocalciferol; Deltalin; Drisdol; Calcidol

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VD2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VD2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of VD2 in the samples is then determined by comparing the OD of the samples to the standard curve.